Genetic Engineering - Cloning, DNA, Stem Cells Pros and Cons
Molecular genetics

The future technology

How To Perform Cloning

First we'll cover a bird's eye view of the basic steps to perform a clone of a cell:

  1. Cut plasmid, insert DNA into plasmid and recombinant plasmidCut a gene at specific regions or anywhere with specialised enzymes know as restriction enzymes.
  2. Cut the vector, carrier (frequently a large harmless virus), usually with the same enzymes you used to cut the gene.
  3. Insert or glue the DNA into the cut vector at the locations the cuts were made
  4. The current state of the process is called "recombinant DNA". Place it into bacteria or yeast.
  5. The yeast or bacteria will grow very fast and in big quantities. Clones will be made from it. They may either contain the vector alone, the vector with the specific foreign DNA, or monster DNA made up of a very strange combination of the vector and me.
  6. Finally, inject the recombinant DNA into a cell. Now, the cell has the DNA piece for the eye colour gene.

To explore the individual steps in detail, view Part 1 of 4 series of steps.

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